Journal: Aging Clinical and Experimental Research

Article Title: Effect of targeted intervention on C-terminal agrin fragment and its association with the components of sarcopenia: a scoping review

doi: 10.1007/s40520-023-02396-w

Figure Lengend Snippet: Association between levels of CAF and secondary sarcopenia

Article Snippet: Bondc et al. 2015 , Effects of a one-year physical activity program on serum C-terminal Agrin Fragment (CAF) concentrations among mobility-limited older adults , To determine the effect of a one-year PA intervention on changes in circulating CAF concentrations and to evaluate association between CAF concentrations and indices of physical function , n = 424. Data from 333 person included in this study Physical activity (PA) and successful aging (SA) arms at 4 sites , Physical activity intervention: PA intervention include walking, strength, flexibility and balance training in center and home-based settings , , Moderate intensity exercise (BORG scale) Asked to walk at target intensity of 13 (somewhat hard) and perform strength training at an intensity of 15 to 16 (hard) , SA intervention: Provide attention and health education, first 26 weeks weekly classes then monthly till end Workshop on topic like nutrition, medication use, foot care and preventive medicine Concluded by short instructor let program of upper extremity stretching exercises , Staff monitored the volume and intensity of exercise by recording the completed walking time and overall RPE each session To encourage participation: regular telephone contact , Primary: 400-m test and SPPB , Stored blood sample at baseline, 6 month and 12 month from a total of 333 participants Serum Commercially available ELISA (NTCAF Elisa Kit; Neurotune, Schlieren, Switzerland) , NA , Gait speed significantly correlated with baseline CAF level ( r = − 0.151, p = 0.006) SPPB not significantly correlated with baseline serum CAF level ( r = − 0.086, p = 0.115) , Significant age*time effect ( p = 0.016): older age was associated with greater increase in serum CAF concentrations from base line to 12 months No significant group*time interaction ( p = 0.265) one-year PA program did not significantly reduce serum CAF levels compared to health education. Sex* time did not suggest sex-related differences in response to intervention ( p = 0.167) Indeed, the standardized beta coefficients for both baseline concentrations of and changes in circulating CAF were small (range: − 0.06 to 0.01) and thus did not significantly contribute to the final model.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Transplantation Assay, Sandwich ELISA, Construct, Marker, Infection, Clinical Proteomics, Battery

Journal: Aging Clinical and Experimental Research

Article Title: Effect of targeted intervention on C-terminal agrin fragment and its association with the components of sarcopenia: a scoping review

doi: 10.1007/s40520-023-02396-w

Figure Lengend Snippet: Effect of interventions (exercise/nutrition/hormonal) on the levels of the CAF

Article Snippet: Bondc et al. 2015 , Effects of a one-year physical activity program on serum C-terminal Agrin Fragment (CAF) concentrations among mobility-limited older adults , To determine the effect of a one-year PA intervention on changes in circulating CAF concentrations and to evaluate association between CAF concentrations and indices of physical function , n = 424. Data from 333 person included in this study Physical activity (PA) and successful aging (SA) arms at 4 sites , Physical activity intervention: PA intervention include walking, strength, flexibility and balance training in center and home-based settings , , Moderate intensity exercise (BORG scale) Asked to walk at target intensity of 13 (somewhat hard) and perform strength training at an intensity of 15 to 16 (hard) , SA intervention: Provide attention and health education, first 26 weeks weekly classes then monthly till end Workshop on topic like nutrition, medication use, foot care and preventive medicine Concluded by short instructor let program of upper extremity stretching exercises , Staff monitored the volume and intensity of exercise by recording the completed walking time and overall RPE each session To encourage participation: regular telephone contact , Primary: 400-m test and SPPB , Stored blood sample at baseline, 6 month and 12 month from a total of 333 participants Serum Commercially available ELISA (NTCAF Elisa Kit; Neurotune, Schlieren, Switzerland) , NA , Gait speed significantly correlated with baseline CAF level ( r = − 0.151, p = 0.006) SPPB not significantly correlated with baseline serum CAF level ( r = − 0.086, p = 0.115) , Significant age*time effect ( p = 0.016): older age was associated with greater increase in serum CAF concentrations from base line to 12 months No significant group*time interaction ( p = 0.265) one-year PA program did not significantly reduce serum CAF levels compared to health education. Sex* time did not suggest sex-related differences in response to intervention ( p = 0.167) Indeed, the standardized beta coefficients for both baseline concentrations of and changes in circulating CAF were small (range: − 0.06 to 0.01) and thus did not significantly contribute to the final model.

Techniques: Control, Marker, Concentration Assay, Activity Assay, Western Blot, Produced, Imaging, Enzyme-linked Immunosorbent Assay, Functional Assay, Modification, Clinical Proteomics